Integrin avidity regulation: are changes in affinity and conformation underemphasized?

Affinity and Avidity of the LFA-1 Integrin is Regulated by Phosphorylation

Academic Dissertation To be present for public criticism, with the permission of 1. LEUKOCYTE FUNCTION-ASSOCIATED ANTIGEN-1 1.1 Introduction 9 1.2 LFA-1 binds to ICAMs 10 1.3 Biology of LFA-1 11 LFA-1 is involved in several fundamental biological processes 11 Leukocyte adhesion deficiency and LFA-1-deficient mice 13 1.4 LFA-1 as a signalling receptor 14 Two-way signalling through LFA-1 14 Intra...

Stabilizing the open conformation of the integrin headpiece with a glycan wedge increases affinity for ligand.

The affinity of the extracellular domain of integrins for ligand is regulated by conformational changes signaled from the cytoplasm. Alternative types of conformational movement in the ligand-binding headpiece have been proposed. In one study, electron micrograph image averages of the headpiece of integrin aV beta 3 show two different conformations. The open conformation of the headpiece is pre...

Chemokines and the Signaling Modules Regulating Integrin Affinity

Integrin-mediated adhesion is a general concept referring to a series of adhesive phenomena including tethering-rolling, affinity, valency, and binding stabilization altogether controlling cell avidity (adhesiveness) for the substrate. Arrest chemokines modulate each aspect of integrin activation, although integrin affinity regulation has been recognized as the prominent event in rapid leukocyt...

Affinity Regulation of the Integrin Avidity 1

Increased extracellular pressure enhances cancer cell integrin binding affinity through phosphorylation of β1-integrin at threonine 788/789

Increased extracellular pressure stimulates β1-integrin-dependent cancer cell adhesion. We asked whether pressure-induced adhesion is mediated by changes in β1-integrin binding affinity or avidity and whether these changes are phosphorylation-dependent. We evaluated integrin affinity and clustering in human SW620 colon cancer cells by measuring differences in binding between soluble Arg-Gly-Asp...